Sumac marc, residue from sumac fruit after oil and flavonoids extraction, was used as a raw material for pyrolysis due to its higher lignocellulosic content in the present work. Time-step and temperature-gradient collections of bio-oil from pyrolysis of sumac mar were established to overcome the disadvantage of high aqueous phase content and low heating value of bio-oil from traditional pyrolysis. In addition, the pyrolysis residues were further applied to prepare activated carbon by chemical activation. Experimental results showed that the aqueous phase content of bio-oil decreased from 94.02% to 28.65% by time-step collection at pyrolysis temperature 500 degrees C with a heating rate 40 degrees C/min in a sweeping gas reactor. In the temperature-gradient collection, the aqueous phase content of bio-oil obtained at 500 degrees C was 15.93% and that gave a promising method to obtain high quality bio-oil preparations from pyrolysis. Under the optimized activation conditions, the iodine and methylene blue absorptivities of activated carbon prepared by pyrolysis residues were 1060 mg/g and 250 mg/g respectively, which were accorded with the international quality standards of first grade activated carbon. Consequently, the pyrolysis of sumac marc to produce bio-oil and preparation of activated carbon from pyrolysis residues provide a new approach of sumac marc application, which achieves a total biomass utilization of sumac marc.