역상 액체 크로마토그래피에 의한 바이온 계면활성제의 Phropylene oxide 분포 및 Fatty alcohol의 분석

이용화; 박홍순; 최규열; 이재덕; 안호정

Journal of the Korean Industrial and Engineering Chemistry, Vol.8, No.2, 315-319, April, 1997

Analysis of Distribution of Propylene oxide in Nonionic Surfactant and Fatty alcohol by Reversed Phase High Performance Liquid Chromatography

이용화, 박홍순, 최규열, 이재덕, 안호정

초록

본 연구는 역상 액체 크로마토그래피를 이용하여 비이온 계면활성제의 propylene oxide 분포 및 고급 지방 알콜의 최적 분석 조건을 조사하였다. Propylene oxide 분포 및 지방 알콜의 탄소 길이를 분석하기 위해 UV 검출기를 사용할 수 있도록 유도체화 시켰으며 최적 분리 조건을 얻기 위해 분리관의 영향과 이동상의 조성 등을 실험하였다. 분리관은 Waters Symmetry C₈(3.9×150mm)을 사용하였고 이동상은 methanol과 물을 이용한 기울기 용리에 의한 분석이 최적 조건이었다. 그리고 이들의 용리 거동은 log k' 와 물의 부피 분율을 도시한 결과 좋은 직선성을 얻었고 고급 알콜의 정량성을 알아보기 위하여 검량선을 도시한 결과 직선성이 좋은 결과를 얻었다.

In this study, the optimum analytical conditions for determination of distribution of propylene oxide in a nonionic surfactant and separation of fatty alcohols were investigated by Reversed Phase High Performance Liquid Chromatography. To analyse the distribution of propylene oxide (PO) and carbon chain length of a fatty alcohol, we derivatized samples for the purpose of using a UV detector. Also, we studied the influences of columns and mobile phase composition to obtain the optimum separation conditions. In our experiment, Waters Symmetry C₈(3.9×150mm) column was used. And the optimum condition were obtained by gradient elution with methanol and water as the mobile phase. In the plot of log k' vs composition of water in the binary phase, the linerality was very good. We ploted the calibration curve to conform the quality of fatty alcohol, a good linerality was obtained.

[References]

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[1] Yoshimura H, Sugiyama T, Nagai T, JAOCS, 64, 550 (1987)

[2] Aserin A, Frenkel M, Garti N, JAOCS, 61, 805 (1984)

[3] Amiji MM, Park K, J. Appl. Polym. Sci., 52(4), 539 (1994)

[4] Mcclure JD, JAOCS, 59, 364 (1982)

[5] Allen MC, Linder DE, JAOCS, 950 (1981)

[6] Kubeck E, Naylor CG, JAOCS, 67, 400 (1990)

[7] Schmitt TM, Allen MC, Brain DK, Guin KF, Lemmel DE, Osburn QW, JAOCS, 67, 103 (1990)

[8] Turner LP, Mccullough D, Jackewitz A, J. Am. Oil Chem. Soc., 53, 691 (1976)

[9] Gildenberg L, Trowbridge JR, J. Am. Oil Chem. Soc., 42, 69 (1964)

[10] Kazuokonishi K, J. Am. Oil Chem. Soc., 51, 55 (1973)

[11] Trathnigg B, Thamer D, Yan X, Kinugasa S, J. Liq. Chromatogra., 16, 2439 (1993)

[12] Trathnigg B, Maier B, Thamer D, J. Liq. Chromatogr., 17, 4285 (1994)

[13] Martin N, J. Liq. Chromatogr., 18, 1173 (1995)

[14] Pasch H, Brinkmann C, Much H, Just U, J. Chromatogr., 623, 315 (1992)

[15] Gorshkov AV, Much H, Becher H, Pasch H, J. Chromatogr., 523, 91 (1990)