In this study, optimization of essential parameters, such as reaction time, reaction temperature, methanol/oil molar ratio, catalyst amount, and type of biodiesel production via transesterification, were investigated. The maximum yield under the optimal conditions was found to be ~95%. Analytical methods were used to determine the fuel characteristics of the final product. The results were compared with specified limits of the ASTM D 6751 and the EN 14214 standards. Finally, produced biodiesel was blended with five different commercial diesel fuels. B20 was found to be an optimum blend without giving any negative effect on petro diesel performance.