Pyridine-2-carboxylic acid (also known as picolinic acid) is used as an intermediate to produce pharmaceuticals, herbicides, and metal salts for applications in the nutritional supplements. Enzymatic oxidation of 3-hydroxyanthranilic acid is a biosynthesis route to produce this acid. Reactive extraction is found to be an attracting separation technique to recover carboxylic acids from their dilute aqueous solutions like fermentation broth, but the toxicity of the extractive system is a major issue. Therefore, an appropriate combination and selection of extractant-diluent system can give a higher distribution of the acid with minimal toxicity. In this paper, the equilibrium study on the reactive extraction of pyridine-2-carboxylic acid [(0.01 to 0.25) kmol.m(-3)] from dilute aqueous solutions is carried out by using extractant-diluent systems consisting of two different extractants, tri-n-butyl phosphate [TBP, (0.365 to 2.192) kmol.m(-3)] and tridodecylamine [TDDA, (0.079 to 0.474) Kmol.m(-3)] and two different diluents, dodecane and (Z)-octadec-9-en-l-ol (also known as oleyl alcohol, OA). The highest value of distribution coefficient (K-D) is found to be 10.11 at lower acid concentration (0.01 Kmol.m(-3)) and higher TDDA (0.474 kmol.m-3) concentration dissolved in OA. The equilibrium constants for the formation of a 1:1 acid-extractant complex are found by employing mass action law and using experimental data. The equilibrium results are also correlated with modified Freundlich, modified Langmuir, and modified Temkin models and fit the Langmuir isotherm model (rmsd = 0.0025) more exactly. The organic phase is regenerated at a high temperature (353 K) with pure water, and its effect is analyzed on the back-extraction efficiency.