In this work, a novel integrated reactor incorporating anoxic fixed bed biofilm reactor (FBBR), oxic moving bed biofilm reactor (MBBR) and settler sequentially was proposed for nitrogen removal from rural domestic sewage. For purposes of achieving high efficiency, low costs and easy maintenance, biomass characteristics and simultaneous nitrification-denitrification (SND) were investigated under long sludge retention time during a 149-day period. The results showed that enhanced SND with proportions of 37.7-42.2% tapped the reactor potentials of efficiency and economy both, despite of C/N ratio of 2.5-4.0 in influent. TN was removed averagely by 69.3% at least, even under internal recycling ratio of 200% and less proportions of biomass assimilation (<3%). Consequently, lower internal recycle and intermittent wasted sludge discharge were feasible to save costs, together with cancellations of sludge return and anoxic stir. Furthermore, biomass with low observed heterotrophic yields (0.053 +/- 0.035g VSS/g COD) and VSS/TSS ratio (<0.55) in MBBR, simplified wasted sludge disposal. (C) 2012 Elsevier Ltd. All rights reserved.