A fibrous bacterial biosorbent was developed to bind precious metal-organic complexes in batch and column processes. Polyethylenimine (PEI)-modified bacterial biosorbent fiber (PBBF) was prepared by spinning Corynebacterium glutamicum biomass-chitosan blends, coating them with PEI and cross-linking with glutaraldehyde. When an acetic acid waste solution containing 1822.9 mg/L Ru was used as a model waste solution, Ru uptake by the PBBF was 16.5 times higher than that of the commercial ion exchange resin, Lewatit MonoPlus M600. The maximum amounts of Ru uptake were 110.5, 16.0 and 6.7 mg/g for PBBF, raw biomass, and Lewatit MonoPlus M600, respectively. In a flow-through packed bed, PBBF exhibited the breakthrough time of 42.32 h. Therefore, PBBF can be considered as an alternative sorbent for recovery of anionic metal-organic complexes from waste solutions. (C) 2012 Elsevier Ltd. All rights reserved.