This study assessed the anaerobic co-digestion of food waste and cattle manure, in order to identify the key parameters that determine the biogas and methane yield. Results of both batch and semi-continuous tests indicated that the total methane production is enhanced in co-digestion, with an optimum food waste (FM) to cattle manure (CM) ratio of 2. At this ratio, the total methane production in batch tests was enhanced by 41.1%, and the corresponding methane yield was 388 mL/g-VS. In the semi-continuous mode, the total methane production in co-digestion, at the organic loading rate (OLR) of 10 g-VSFW/L/d, increased by 55.2%, corresponding to the methane yield of 317 mL/g-VS. Addition of cattle manure enhanced the buffer capacity (created by NH4+ and VFAs), allowing high organic load without pH control. The C/N ratio and the higher biodegradation of lipids might be the main reasons for the biogas production improvement. (C) 2012 Elsevier Ltd. All rights reserved.