Crude oil is an extremely complex mixture of hydrocarbons; also contaminate environmental, leading to carcinogenic, teratogenic and mutagenic. Petroleum hydrocarbons degradation Dietzia strain DMYR9 was isolated from oilfield. Response surface methodology was applied for statistical designing of process parameters for dry weight of biomass production in the process of degradation. The optimization process parameters were successfully employed for degradation crude oil and confirmed through confirmatory experiments. On 28th day, analysis was done by GC MS, These data show that the crude oil samples of n-Hexadecane, Octadecane, n-Nonadecanec, n-Pentacosane, n-Hexacosane, n-Heneicosane, n-Docosane, n-Tetracosane, n-Octacosane and Tetraethyl removal efficiency could reach up to 0%. RSM optimization and use of effective C-12(6+)-ion irradiation methods can considerably enhance ability to degradation of microbial. Hence, bioresource Dietzia strain DMYR9, high ability to degradation, can be further used for subsequent repair hydrocarbons polluted of environment. (C) 2013 Elsevier Ltd. All rights reserved.