Succinic acid is an important precursor for the synthesis of high-value-added products. Saccharomyces cerevisiae is a suitable platform for succinic acid production because of its high tolerance towards acidity. In this study, a modified pathway for succinate production was established and investigated in S. cerevisiae. The engineered strain could produce up to 6.17 +/- 0.34 g/L of succinate through the constructed pathway. The succinate titer was further improved to 8.09 +/- 0.28 g/L by the deletion of GPD1 and even higher to 9.98 +/- 0.23 g/L with a yield of 0.32 mol/mol glucose through regulation of biotin and urea levels. Under optimal supplemental CO2 conditions in a bioreactor, the engineered strain produced 12.97 +/- 0.42 g/L succinate with a yield of 0.21 mol/mol glucose at pH 3.8. These results demonstrated that the proposed engineering strategy was efficient for succinic acid production at low pH value. (C) 2014 Elsevier Ltd. All rights reserved.