Biological nitrogen removal in aerobic granular sequencing batch reactors is sensitively affected by process conditions (e.g. dissolved oxygen (DO) concentration, nitrogen loading rate (NLR), influent C/N ratio, among others). The variation of one of these process conditions affects the others, because often they are tightly linked. These interrelationships are a drawback for the experimental assessment of the target domain of process conditions required to enhance N-removal. Here, we have developed a model to determine the guidelines to design an automatic control strategy with the final aim of enhancing biological N-removal in a granular sequencing batch reactor. The model was first calibrated with experimental data from a granular sequencing batch reactor treating swine wastewater. Specific simulations were designed to elucidate the effect of DO concentration (0.5-8 mg O-2 L-1), granule size (0.5-3.5 mm), influent C/N ratio (4-10 g O-2 g(-1) N) and NLR (0.41-0.82 g N L-1 d(-1)) on the nitrification-denitrification efficiency. Simulation results showed that, in general, high N-removal efficiencies (from 70% to 85%) could be obtained only setting the appropriate DO concentration. That appropriate DO concentration could be easily found based on effluent ammonium concentration. Those results were used to propose a control strategy to enhance N-removal efficiencies. The control strategy was based on a closed DO loop with variable DO set-point. The DO set-point was established at a constant value for the whole cycle (i.e. once per cycle), based on the on-line measurement of ammonium concentration at the end of the previous cycle. (C) 2013 Elsevier B.V. All rights reserved.