Chemical Engineering Science, Vol.97, 385-393, 2013
Parameters influencing magnetically enhanced centrifugation for protein separation
Magnetically Enhanced Centrifugation is a hybrid method combining the advantages of magnetic separation and centrifugation. Magnetic particles are separated due to magnetic wire forces to a wire matrix within the centrifuge. The deposit slides off the matrix by centrifugation. A pilot machine was set up for batch-wise separation. The discharge takes place by resuspending the particles using the matrix as a stirrer. The influence of the volumetric flow rate, magnetic field strength, and particle concentration is investigated. 200 g of magnetic particles are filled in the device. A separation efficiency of more than 99% is possible for the separation of synthetic particles. Concerning discharge, 93% of 200 g particles can be recovered in 2 batch volumes of 1.2 I of liquid each. In further experiments, a model protein particle system using hen egg white provides a case study for protein separation by Magnetically Enhanced Centrifugation. (C) 2013 Elsevier Ltd. All rights reserved.
Keywords:Centrifugation;Adsorption;Ion exchange;Particulate process;Magnetic Separation;Hybrid process