The comments of Plekan et al. (2013) on the Letter Valence ionization of L-proline amino acid: experimental and theoretical study, Chem. Phys. Lett. 565 (2013) 102 ' are entirely replied to. We strongly confirm our experimental and theoretical results on the photoelectron spectroscopy of L-proline in our published Letter. (C) 2014 Elsevier B.V. All rights reserved.