부분 가수분해된 수용성 키토산의 제조와 그 항균성에 관한 연구

김천호; 이응석; 함영태; 김병용; 손태일

Journal of the Korean Industrial and Engineering Chemistry, Vol.10, No.1, 19-23, February, 1999

Export Citation

부분 가수분해된 수용성 키토산의 제조와 그 항균성에 관한 연구

Synthesis of Partially Hydrolyzed Water-soluble Chitosans and their Antibacterial Activities

김천호, 이응석, 함영태, 김병용, 손태일

초록

아질산염을 이용한 키토산의 부분가수분해 반응에서 반응이 진행됨에 따라서 탈아세틸화도는 감소하였으며 가수분해된 각 fraction에서의 탈아세틸화도는 50% 이하로 확인되었다. GPC를 이용하여 이들 각 유도체의 수평균분자량을 확인한 결과, 물-메탄올에 의한 침전물은 6000에서 4000의 분자량을 가지고 있었으며, 메탄올-아세톤에 의해서는 2000 이하의 분자량을 가지는 것을 얻을 수 있었다. 이때 다분산도는 1.7 이하로 분자량 분포가 좁은 분해물을 얻을 수 있었다. 각 fraction별로 분자량에 따른 항균효과를 E. coli HB 101과 Bacillus subtilis PP 2에 대해서 시험한 결과 분자량이 5100인 fraction B(H₂O : MeOH = 1:5)가 가장 우수하였고, 모든 시료에서 그람양성균인 Bacillus subtilis PP 2에 대한 항균능이 그람음성균인 E. doli HB 101에 비해 우수하였다.

Chitosan was partially degraded by using nitrous acid. The deacetylation degree of chitosan decreased with its degree of hydrolysis. Deacetylation degree of each fraction was less than 50%. The degraded product was fractionated by means of precipitation using aqueous methanol solution or aqueous methanol-acetone solution. The molecular weight of each fraction was distributed between 6000 and 4000, and below 2000 for being precipitated using aqueous methanol solution and aqueous methanol-acetone solution respectively. They had narrow molecular weight distributions, and their polydispersities were less than 1.7. The antibacterial activities for each fraction were evaluated against Bacillus subtilis HB 101 and E. coli PP 2, gram-positive bacteria and gram-negative bacteria, respectively. Fraction B (Mw=5100) showed high antibacteiral activity. All fractions were more active against Bacillus subtilis than E. coli.

Keywords:Bacillus Subtilis;E. coli;GPC;Depolymerization;Nitrous Acid

[References]

Muzzarelli RAA, "Chitin," Pergamon Press, Oxford (1977)
Hirano S, Noisiki Y, J. Biomed. Mater. Res., 19, 413 (1985)
Kojima K, Yoshikuni M, Suzuki T, J. Appl. Polym. Sci., 24, 1587 (1979)
Allan CR, Hadwiger LA, Exp. Mycol., 3, 285 (1979)
Kendra DF, Hadwiger LA, Exp. Mycol., 8, 276 (1984)
Uchida Y, Food Chem., 2, 22 (1988)
Seo H, Mitsuhashi K, Tanibe H, Advances in Chitin and Chitosan, eds. C.J. Brine, P.A. Sandford and J.P. Zikakis, p. 34, Elsevier Applied Scienced, London (1992)
Hadwiger LA, Chitin in Nature and Technology, eds. by R.A.A. Muzzarelli, C. Jeuniaux and G.W. Gooday, p. 209, Plenum Press (1986)
Kim CH, Kim SY, Choi KS, Polym. Adv. Technol., 8, 319 (1997)
Kim CH, Choi JW, Chun HJ, Choi KS, Polym. Bull., 38(4), 387 (1997)
Kina K, Tamura K, Ishibashi N, Jpn. Anal., 23, 1082 (1974)
Doson CR, Frank HA, Cavaietto CG, J. Food Sci., 42, 273 (1977)
Allan GG, Peyron M, Proceedings from the 4th International Conference on Chitin and Chitosan, eds. by G. Skjak-Braek, T. Anthonsen and P. Stanford, p. 443, Norway (1988)
Kawabata N, Kobunji Kako, 34, 583 (1985)
Franklin TJ, Snow GA, Biochem. of Antimicrobial. Action, Chapter 3, Chapman & Hall, London (1981)

[Referenced By]

[1] Muzzarelli RAA, "Chitin," Pergamon Press, Oxford (1977)

[2] Hirano S, Noisiki Y, J. Biomed. Mater. Res., 19, 413 (1985)

[3] Kojima K, Yoshikuni M, Suzuki T, J. Appl. Polym. Sci., 24, 1587 (1979)

[4] Allan CR, Hadwiger LA, Exp. Mycol., 3, 285 (1979)

[5] Kendra DF, Hadwiger LA, Exp. Mycol., 8, 276 (1984)

[6] Uchida Y, Food Chem., 2, 22 (1988)

[7] Seo H, Mitsuhashi K, Tanibe H, Advances in Chitin and Chitosan, eds. C.J. Brine, P.A. Sandford and J.P. Zikakis, p. 34, Elsevier Applied Scienced, London (1992)

[8] Hadwiger LA, Chitin in Nature and Technology, eds. by R.A.A. Muzzarelli, C. Jeuniaux and G.W. Gooday, p. 209, Plenum Press (1986)

[9] Kim CH, Kim SY, Choi KS, Polym. Adv. Technol., 8, 319 (1997)

[10] Kim CH, Choi JW, Chun HJ, Choi KS, Polym. Bull., 38(4), 387 (1997)

[11] Kina K, Tamura K, Ishibashi N, Jpn. Anal., 23, 1082 (1974)

[12] Doson CR, Frank HA, Cavaietto CG, J. Food Sci., 42, 273 (1977)

[13] Allan GG, Peyron M, Proceedings from the 4th International Conference on Chitin and Chitosan, eds. by G. Skjak-Braek, T. Anthonsen and P. Stanford, p. 443, Norway (1988)

[14] Kawabata N, Kobunji Kako, 34, 583 (1985)

[15] Franklin TJ, Snow GA, Biochem. of Antimicrobial. Action, Chapter 3, Chapman & Hall, London (1981)