This work describes the electrochemical behavior of Ni(II)-pyromellitic acid (PMA) film immobilized on the surface of bimetallic Au-Pt inorganic-organic hybrid nanocomposite carbon nanotube glassy carbon electrode and its electrocatalytic activity toward oxidation of glucose. The surface structure and composition of the sensor was characterized by scanning electron microscopy (SEM). Electrocatalytic oxidation of glucose on the surface of modified electrode was investigated with cyclic voltammetry and rotating disk electrode (RDE) voltammetry methods. The results showed that nickel pyromellitic acid films on bimetallic Au-Pt inorganic-organic hybrid nanocomposite carbon nanotube glassy carbon electrode have excellent electrochemical catalytic activity toward glucose oxidation. The hydrodynamic amperometry at rotating modified electrode at constant potential versus reference electrode was used for detection of glucose. Under optimized conditions, the calibration plots are linear in the concentration range of 100 nM to 100 mu M and detection limit was found to be 55 nM. The modified electrode showed reproducible behavior and a high level of stability during electrochemical experiments, making it particularly suitable for analytical purposes. (C) 2012 Elsevier Ltd. All rights reserved.