A novel amperometric sensor based on silver nanowire (Ag NW) array was fabricated and used for a rapid and sensitive detection of hydrogen peroxide (H2O2). The 50-nm in diameter nanowires were synthesized by electrodeposition of silver in the nanopores of anodic aluminum oxide (AAO) template. At an applied potential of -0.2V vs. SCE, the developed Ag NW array electrode exhibited electrocatalytic response toward the reduction of H2O2 at a very wide range of H2O2 concentrations and three significantly linear sections in a calibration plot were observed. The determination of H2O2 with the highest sensitivity of about 0.0266 mu A cm(-2) mu M-1 is possible in the concentration range from 0.1 mM to 3.1 mM with a detection limit of 29.2 mu M. Moreover, the proposed sensor exhibited relatively high sensibility, good reproducibility, and satisfying long-term stability. The developed Ag NW array sensor excluded the interference from substances typically present in the biological samples such as ascorbic acid (AA), uric acid (UA), ethanol (EtOH), glucose (G) and oxalate ions. (C) 2013 Elsevier Ltd. All rights reserved.