Biofuel cells are important to generate alternative energy. This paper evaluates the electroactivity of a carbon cloth platform containing immobilized multiwalled carbon nanotubes (MWCNTs), a NAD(+)-dependent alcohol dehydrogenase enzyme (ADH), poly-methylene green (poly-MG), and an anchoring agent (PAMAM dendrimer, modified NAFION (m-NAFION), or polypyrrole (poly-Pyr)). The PAMAM dendrimer is an excellent medium to achieve ADH immobilization on the carbon nanostructured platform. We obtained power density and open circuit potential (OCP) of 0.278 +/- 0.038 mW cm(-2) and 0.459 +/- 0.025 V, respectively, for PAMAM modified with MWCNTs. This new anode configuration is potentially applicable for small energy devices. (c) 2013 Elsevier Ltd. All rights reserved.