AFCs (alkaline fuel cells) is one of the promising fuel cells, due to their low working temperature and less corrosive environment. However, decreasing the catalyst cost and improving its performance are still the challenges in its application. Transition metal as the catalyst for AFCs not only can reduce its cost, but also has great electro-catalytic efficiency. In this paper, Carbon supported Ag-Ni bimetallic catalysts with differential Ag/Ni atomic ratios were prepared by chemically reducing silver and nickel salts. Ag3Ni/C shows the relatively higher ORR (oxygen reduction reaction) activity among the differential Ag/Ni bimetallic particles. In order to improve the activity and stability, the catalysts were heat-treated at the temperature of 500 C. The results indicate that the limiting current density has been improved greatly for Ag3Ni/C-500 degrees C, which is as high as 2.5x that of Ag/C. The microstructure investigation show that the non-equilibrium state of Ag-Ni alloy by heat treatment is confirmed by HRTEM (high-resolution transmission electron microscopy) images, and Ag(111) surfaces are decreased in XRD pattern, which results in the ORR activity improved and overpotential decreased. Heat treatment also has contributed to Ag Ni/C electrochemistry stability in some degree. (C) 2014 Elsevier Ltd. All rights reserved.