Polyurethane (PU) adhesives for wood bonding were prepared from palm oil-based polyester polyol in a solventless condition that reduces the risk of volatile organic compounds to human health and the environment. The polyester polyol was synthesized from epoxidized palm olein prior to reacting with polymeric 4,4 '-methylene diphenyl diisocyanate (pMDI) and toluene 2,4-diisocyanate (TDI) to produce wood-bonding PU adhesives. The effect of glycerol cross-linker, dibutyltin dilaurate catalyst and NCO/OH ratio on lap shear strength and pot life of the PU adhesives were studied. The green strength of the PU adhesives was achieved on day 4 for TDI-based adhesives and day 5 for pMDI-based adhesives. The newly formulated PU adhesives have superior chemical resistance in cold water, hot water, acidic medium and alkaline medium by only showing light deterioration (2-8%) in lap shear strength. The PU adhesives prepared from pMDI exhibited higher lap shear strength and thermal stability as compared to adhesives prepared from TDI adduct. Both adhesives have improved mechanical performance (two folds higher in lap shear strength) as compared to commercial wood bonding adhesives.