Conventional biodiesel preparation either requires the use of catalyst such as potassium hydroxide and sulfuric acid or carries out methanolysis under supercritical methanol condition without the need of catalyst. In this study, a new method for biodiesel production was proposed. Biomass of the oleaginous yeast Yarrowia lipolytica Po1g was reacted with methanol and a small amount of water under subcritical condition (175 degrees C, 23 bar) without the presence of acid or base. The proposed method was able to give high biodiesel yield in reasonably short time. The possibility of using this method for biodiesel preparation from wet biomass with high acid and water contents as feedstock was discussed. (C) 2013 Elsevier B.V. All rights reserved.