Hydrogen internal combustion engine (H2ICE) easily occur inlet manifold backfire and other abnormal combustion phenomena because of the low ignition energy, wide flammability range and rapid combustion speed of hydrogen. In this paper, the effect of injection timing on mixture formation in a manifold injection H2ICE was studied in various engine speed and equivalence ratio by CFD simulation. It was concluded that H2ICE of manifold injection have an limited injection end timing in order to prevent backfire in the inlet manifold. Finally, the limit of injection end timing of the H2ICE was proposed and validated by engine experiment. (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.