생분해성 고분자 마이크로겔 Microsphere의 약물방출 특성 및 항암효과

김공수; 김정목; 김태경; Kong Soo Kim; Jung Mook Kim; Tae Kyung Kim

Journal of the Korean Industrial and Engineering Chemistry, Vol.11, No.2, 133-137, April, 2000

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생분해성 고분자 마이크로겔 Microsphere의 약물방출 특성 및 항암효과

Drug Release Behaviors and Antitumor Effects of The Biodegradable Microgel Microspheres

김공수, 김정목^†, 김태경

충북대학교 공과대학 화공학부, 청주 48

Kong Soo Kim, Jung Mook Kim^†, and Tae Kyung Kim

Dept. of Indust. Chem., Chungbuk Nat'l Univ., Chungju 48, Korea

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초록

폴리카프로락톤을 기초로 하여 용액증합방법으로 microgel을 합성하고, 용매증발법으로 모델 약물인 BSA와 5-fluorouracil (5-FU)이 담지된 microsphere를 제조하였다. Microgel을 제조함에 있어 가교제의 양과 pH 및 BSA와 5-FU의 담지량에 따른 약물의 방출속도를 in vitro 실험한 결과, BSA를 함유하는 microsphere가 5-FU를 함유하는 경우 보다 약물이 훨씬 빨라 방출되었다. 또한, 5-FU를 담지한 microsphere를 saecoma-180 asites 종양세포가 이식된 마우스에 투여하여 실험한 결과, 투여량에 따라 암종양세포에 대하여 현저한 연명효과를 나타내었다.

Biodegradable microsphere containing Bovine Serum Albumin (BSA) or 5-fluorouracil (5-FU) was prepared with the microgel based on poly(caprolactone diol) by a modified solvent evaporation method. The effects of the crosslinking degree of microgel, pH, the concentration of BSA and 5-FU of medium on the release properties were investigated in vitro. Drug release rate from the micorsphere containing BSA was relatively rapid than the microsphere containing 5-FU. Antitumor activities were tested against Sarcoma-180 asites in vivo. The rate of medium survival of treated mice to that of control mice was depend on injected amount of the microsphere containing 5-FU.

Keywords:microgel;microsphere;drug delivery system;antitumor activity

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[5] Graham NB, Brit. Polym. J., 10, 260 (1978)

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[16] Kim KS, Graham NB, J. Ind. Eng. Chem., 4(3), 221 (1998)

[17] Kim KS, Kang SH, Kim JM, J. Korean Ind. Eng. Chem., 9(4), 591 (1998)