Due to the energy crisis and global warming issues, the development of clean alternative energy is in urgent demand. Biohydrogen produced from cellulosic feedstock is a promising candidate as a clean energy carrier to meet the future needs. This article produced biohydrogen from various cellulosic materials via one-step process. The feedstocks applied included glucose, cellobiose, carboxymethyl cellulose (CMC), Whatman No. 2 filter paper, lignin, xylan, and two microalgae samples. Enriched inoculum was applied for biohydrogen tests. The present bacterial consortium can produce biohydrogen via one-step process. Dilution-to-extinction approach was adopted to identify the functional consortium for simultaneous hydrolysis and fermentation of filter papers. Copyright (c) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.