Crude glycerol (CG) has several advantages over a range of conventional substrates used for biohydrogen production by Enterobacter aerogenes NRRL B 407. Meanwhile, high process cost due to requirement of expensive supplementary media component is a concern. Therefore, different less expensive (or wastes) materials have been evaluated as supplementary nutrient for H-2 production by CG (meat processing and restaurant waste based biodiesel derived) bioconversion. Among the materials selected, slaughterhouse liquid waste (SL), brewery waste biomass (BWB) and urea was found to improve the production by 18.81 +/- 3.56, 27.30 +/- 3.54 and 38.57 +/- 3.66%, respectively. Further, in the case of urea (10 mg/L), cumulative production as high as 116.41 +/- 3.72 mmol H-2/L media has been achieved; which is comparable to other reports available on CG bioconversion. Thus, present study demonstrates successful replacement of large amount (similar to 5-6 g/L) of expensive nutrients/buffering agents by negligible amount (similar to 10 mg/L) of different waste materials, without compromising the cumulative H-2 yield. Further, the strain used in the present study was found to grow at an acidic pH as low as 3.3, indicating its prospective application for dark fermentative H-2 production. Copyright (C) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.