Nafion hybrid membrane containing titanium dioxide (TiO2) nano-particles were elaborated by in situ sol-gel technique. Micro wave treatment was used during hybrid elaboration to 2 h. The X-ray diffraction (XRD) shows the crystallization of TiO2 in anatase and rutile phases. The Infrared spectroscopy IR spectrum of hybrid membranes Nafion-TiO2 confirm the presence of bonds Ti-O and Ti-OH which proves that the hybridization of the membrane by sol-gel method is successful. Atomic Force Microscopy AFM confirmed the good dispersion of TiO2 nano-particles. The electrochemical impedance spectroscopy (EIS) and solvent uptake measurements suggest the enhancement of the membrane performance. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.