Electrochemical Impedance Spectroscopy (EIS) is a suitable and powerful diagnostic testing method for fuel cells because it is non-destructive and provides useful information about fuel cell performance and its components. In this work, EIS measurements were carried out on a 300 W stack with 20 elementary cells. Electrochemical impedance spectra were recorded either on each cell or on the stack. Parameters of a Randles-like equivalent circuit were fitted to the experimental data. In order to improve the quality of the fit, the classical Randles cell was extended by changing the standard plane capacitor into a constant phase element (CPE). The effects of output current, cell position, operating temperature and humidification temperature on the impedance spectra were studied. Copyright (C) 2013, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.