Korea Polymer Journal, Vol.2, No.1, 32-39, April, 1994
Protein Behavior on Polymer surfaces with Hydrophilic Functional Groups
Surfaces with hydrophilic functional groups were prepared on low density polyethylene(PE) sheets by corona discharge treatment, followed by graft copolymerization of acrylic acid (-COOH, negatively chargeable) or acryl amide (-CONH2, neutral). The prepared surfaces were characterized by the measurement of water contact angle, electron spectroscopy for chemical analysis (ESCA) and Fourier-transform infrared spectroscopy in the attenuated total reflectance mode (FTIR-ATR). It was observed that the wettability of the functional group-grafted surface increased largely compared to control surface but was not much affected by the kind of grafted functional groups (-COOH and -CONH2 groups). The adsorption and desorption behavior of blood proteins such as albumin, γ-globulin, and fibrinogen on the control (hydrophobic) and the functional group-grafted (hydrophilic) surfaces was investigated in this study. The control PE surface showed large adsorption and desorption of the proteins due to the hydrophobic character. For the functional group-grafted surfaces the surface grafted with -COOH groups showed less protein adsorption than that grafted with -CONH2 groups probably due to the negatively chargeable character in aqueous protein solution. Among the proteins used, fibrinogen was adsorbed most largely on the surfaces. It may be due to its unusually long three-dimensional structure with high molecular weight.
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