This paper represents the results of an experimental study on the flow structure around a single sphere and three spheres in an equilateral-triangular arrangement. Flow field measurements were performed using a Particle Image Velocimetry (PIV) technique and dye visualization in an open water channel for a Reynolds number of Re = 5 x 10(3) based on the sphere diameter. The distributions and flow features at the critical locations of the contours of the velocity fluctuations, the patterns of sectional streamlines, the vorticity contours, the turbulent kinetic energy, the Reynolds stress correlations and shedding frequency are discussed. The gap ratios (G/D) of the three spheres were varied in the range of 1.0 <= G/D <= 2.5 where G was the distance between the sphere centers, and D was the sphere diameter which was taken as 30 mm. Due to the interference of the shedding shear layers and the wakes, more complex features of the flow patterns can be found in the wake region of the two downstream spheres behind the leading sphere. For G/D = 1.25, a jet-like flow around the leading sphere through the gap between the two downstream spheres occurred, which significantly enhanced the wake region. It was observed that a continuous flow development involving shearing phenomena and the interactions of shedding vortices caused a high rate of fluctuations over the whole flow field although most of the time-averaged flow patterns were almost symmetric about the two downstream spheres. (C) 2013 Elsevier Ltd. All rights reserved.