A laser diffraction particle sizing method involving wet analysis Could be adapted effectively to measure the accurate particle size distribution of a spray-dried infant formula. Polar, polar aprotic and non-polar solvents, such as ethanol, methanol, acetone, pentane, heptane and hexane, were tested as dispersants for wet analysis. Non-polar solvents such as pentane, heptane and hexane found to be suitable dispersant because the shape of the infant formula particles in non-polar solvents was similar before and after the measurement while the particles had dissolved in the other solvents. The particle size distributions (PSD) of the infant formula determined by laser diffraction (Malvern Master Sizer, UK) using the dry analysis method with air was unsuitable because some parts of the primary and aggregated infant formula particles had been destroyed. The PSD graph of the air dispersion was shifted toward a smaller particle size from that of hexane dispersion. Overall, it is believed that laser diffraction particle sizing involving wet analysis with non-polar solvents may provide a suitable particle sizing method for infant formula products that is better than an air dispersion method. (C) 2008 Elsevier Ltd. All rights reserved.