Ultrasonic longitudinal attenuation and velocity have been measured for various frequencies between 05 and 135 MHz versus temperature in honeys with moisture contents between 15% and 19% With the help of attenuation measurements the temperature of the alpha transition T(alpha) (associated with the glass transition) has been deduced versus moisture content and ultrasonic frequency Regarding the high sensitivity of T(alpha), towards moisture content one can distinguish two honeys with moisture contents difference less than 01% Considering the fact that the experiments are not difficult to perform and are carried out at around room temperature this technique could be useful for honey quality control or more generally for aqueous polymers study (C) 2010 Elsevier Ltd All rights reserved