Palm kernel cake contains residual oil that has never been retrieved and it is treated as wastes. In this study, supercritical carbon dioxide was used to separate the oil from the palm kernel matrix. The studied extraction parameters were at the set pressures 27.57, 34.47 and 41.36 MPa, temperatures 40-70 degrees C and carbon dioxide flow rate between 1 and 3 ml/min. The effect of the particle size on the oil yield was examined using different particle size that separated by sieving from <= 106, <= 150, <= 180, <= 250 and <= 450 mu m. The results showed that the highest oil removed was 9.26 g oil/100 g sample (p < 0.05) for the particle <= 150 mu m, and under extraction temperature of 70 degrees C, pressure 41.36 MPa, and carbon dioxide flow rate of 2 ml/min. The experimental results indicate that supercritical carbon dioxide extraction could be a viable technique to remove the remaining oil and to produce defatted palm kernel cake. (C) 2011 Elsevier Ltd. All rights reserved.