Kokum kernel is a byproduct of agro-processing industry in India containing about 40-50% fat which has the potential as a worthy cocoa butter alternative (CBA). However, inefficient extraction techniques that are practiced at cottage level restrict its industrial applications. This work reports on the optimization of the technique of three phase partitioning (TPP) for efficient extraction of kokum kernel fat. The parameters of TPP were optimized with respect to ammonium sulphate concentration, ratio of slurry to t-butanol and pH of slurry. The optimized protocol resulted in maximum recovery of 95% (w/w) fat recovery within 2 h. The technique is economical and eco-friendly, and is promising for utilization of agro-processing waste in India to a product of commercial significance. (C) 2012 Elsevier Ltd. All rights reserved.