Electrolyzed oxidizing (EO) water has been recommended to be used as a cleaning and sanitizing agent for Clean-In-Place (CIP) of on-farm milking systems. The CIP process for milking system with EO water was optimized using a pilot-scale pipeline milking system. The milking system was soiled using raw milk inoculated with four common microorganisms found in milk and cleaned using EO water. The cleaning effectiveness of the EO water treatment was evaluated by ATP bioluminescence test and microbiological analysis through enrichment culture. The effect of different temperatures of the alkaline ED water (45-75 degrees C) and acidic EO water (25-45 degrees C) were investigated. A generalized mathematical model was built and validated for the pilot scale milking system as well as compared with the conventional CIP technique. This study indicated that the EO water CIP outperformed the conventional CIP, indicating that it has a potential to be used in commercial dairy farms. (C) 2014 Elsevier Ltd. All rights reserved.