The bioaccumulation and depuration of six hexabromocyclododecane enantiomers in zebrafish were investigated through dietary exposure. The bioaccumulation of (+/-)alpha-HBCD, (+/-)beta-HBCD and (+/-)gamma-HBCD was concentration dependent. The results from their concentration trends and assimilation efficiencies suggested that zebrafish selectively bioaccumulate the (+) alpha-enantiomer and (+) gamma-enantiomer compared to their corresponding (-)-enantiomers. However, (+/-)beta-HBCD did not show enantioselectivity in the process of bioaccumulation. The deputation of all six enantiomers in zebrafish followed first-order kinetics except (-)gamma-HBCD in low dose. These results showed that the bioaccumulation and deputation of HBCDs in zebrafish were enantiomer-selective. Each enantiomeric pair of HBCDs could display different biological process in organisms. (C) 2013 Elsevier B.V. All rights reserved.