Humic substance (HS) in sediment can affect hydrophobic organic compound distribution, transportation, bioavailability, and toxicity. This study investigated the HS (BKHS) extracted from sediment and separated it into low molecular humic (LMHS, <1 kDa) and high molecular humic substances (HMHS, 1 kDa-0.45 mu m). Nonylphenol (NP), octylphenol (OP), and bisphenol A (BPA) have a significant sorption capacity for HMHS and BKHS solutions. They are xenoestrogenic endocrine-disrupting compounds that are widely produced and discharged to the environment. The log K-HS values of the BKHS and HMHS solutions were between 4.74-5.09 L kg-C-1 and 4.57-5.09 L kg-C-1, respectively. However, the three compounds were not sorbed by the LMHS solution. The average values of SUVA(254) for HMHS and LMHS were 4.29 and 1.31 Lm(-1) mg-C-1 and the average values of A(250-400) for HMHS and LMHS were 18.1 and 4.51 nm cm(-1), respectively. The HMHS peak position in the fluorescence excitation/emission matrix at longer wavelengths corresponded to the peak position of LMHS, which indicates that the HMHS had a higher degree of humification than the LMHS. The results suggested that the Km value's dominant factor was the degree of HS humification. (C) 2014 Elsevier B.V. All rights reserved.