A full scale anaerobicanoxicoxic (A(2)O) biological unit followed by a submerged MBR was applied for domestic wastewater treatment and reclaimed water production. Under a constant flux of 16 L/m(2)h, TMP varied narrowly between 12 and 22 KPa over the duration of 9 months with only enhanced flux maintenance (EFM) for restoring membrane permeability. By analyzing the particle size distribution (PSD) and extracellular substances (EPS) in the mixed liquor at different steps, it was found that fine particles, proteins and polysaccharides, as potential foulants, decreased along the A(2)OMBR array. Fluorescent excitationemission matrix (3DEEM) analysis further revealed the characteristics of dissolved organic substances throughout the treatment process. Nevertheless, the deposition of organic foulants was identified in the cake layer on the membrane surface by scanning electron microscopy and Fourier transform infrared spectroscopy. The advantage of the A(2)OMBR system over the conventional MBR was not only in the effectiveness for high quality reclaimed water production, but also in the reduction of potential foulants through the A(2)O biological process prior to entering the MBR unit.