The lipase-catalyzed synthesis of n-butyl lactate by esterification was performed in supercritical trifluoromethane. Immobilized lipase B from Candida antarctica (Novozym 435) was used as a biocatalyst. Process conditions (pressure and temperature) were optimized performing experiments in a high-pressure batch stirred-tank reactor. Experiments were carried out in the operative pressure ranges from 7.5 to 30 MPa and at temperatures 35 degrees C and 55 degrees C. For this purpose phase behavior for D,L-lactic acid/n-butanol/Novozym 435/supercritical fluid system at temperature 55 degrees C and different pressures was studied. The highest conversion of lactic acid after 26 h of reaction performance was obtained in supercritical trifluoromethane at 30 MPa and 55 degrees C. The n-butyl lactate (standard and enzymatically synthesized) and DJ-lactic acid were tested against four food-borne fungi: Saccharomyces cerevisiae, Aspergillus niger, Trichoderma viride and Penicillium cyclopium and three health-damaging bacteria: Escherichia coil, Pseudomonas fluorescens and Bacillus cereus by the agar well diffusion. (C) 2013 Elsevier B.V. All rights reserved.