We describe a simple method for the simultaneous determination of association constants for a guest binding to seven different hosts in a mixture of more than 20 different oligosaccharides. If the binding parameters are known for one component in the mixture, a single NMR titration suffices to determine binding constants for all other detectable and resolvable hosts. With the use of high-resolution H-1-C-13 HSQC experiments, complexes of amphiphiles with more than 10 different maltooligosaccharides can be resolved. Hereby, the binding capabilities of a set of structurally related hosts can be quantitatively studied to systematically explore noncovalent interactions without the need to isolate each host.