Maltodextrin with dextrose equivalence between 4 and 7 was proposed for the design of a multimode sensor. The multimode term was introduced for the sensors that can be used in more than one electrochemical method. The selection of the materials was based on its nanostructure nature (for stochastic mode), and its catalytic activity (for amperometric mode). The qualitative analysis was performed using the stochastic mode, while the quantitative analysis of the antigens was performed using both stochastic and amperometric modes. The biomarkers specific for hepatitis B and breast cancer were determined directly from the biological fluids (e.g., whole blood). The response characteristics of the new multimode sensors for CA15-3, and hepatitis B biomarkers (HBV antigen and D-pipecolic acid) (linear concentration ranges, limits of determination) as well as their recovery tests performed using whole blood samples proved that they can be used for screening tests of whole blood with high reliability. (C) 2014 The Electrochemical Society. All rights reserved.