A novel nanocomposite of gold nanoparticles/neutral red/MWCNTs was prepared which was used to modify glassy carbon electrode. The prepared nanocomposite was physically characterized by scanning electron microscopy, transmission electron microscopy, zeta potential measurement, energy dispersive X-ray, FTIR spectroscopy, UV-visible spectroscopy. Electrochemical characterization was done using cyclic voltammetry technique. The modified glassy carbon electrode showed electrocatalytic activity toward the oxidation of NADH in 0.1 M phosphate buffer solution, pH 5.0. The modified electrode has better adhesion over the electrode surface, good stability as no leaching of neutral red based nanocomposite was observed. The oxidation of NADH started at 0.37 V and reached maxima at 0.52 Vat the modified electrode surface. So the prepared composite modified electrode can be applied as electrochemical sensor for NADH. The sensitivity and detection limits of the modified glassy carbon electrode were found to be 0.588 mu A/mM and 5 x 10(-7) at signal to noise ratio 3. (C) 2013 Elsevier Ltd. All rights reserved.