Magnetic Barkhausen noise measurements have been carried out to characterize ferrite-martensite duplex microstructures and industrial Dual-Phase steels. We have first studied ferrite-martensite duplex steels, for which the volume fraction and the carbon content of martensite were higher than for industrial Dual-Phase steels. We found linear evolutions between ferrite peak parameters and its proportion. We applied these results to industrial Dual-Phase steels and show that Barkhausen noise measurement can be successfully used for Dual-Phase steels characterization, and in particular for assessment of ferrite proportion.