It is reasonable to speculate the adaptability of Horio's scaling law (equivalent to the simplified Glicksman's scaling law) to the particle mixing and segregation behavior in bubbling fluidized bed (BFB), because the Horio's scaling law is derived from the governing equations of bubbles, and the particle mixing and segregation are dependent on bubble dynamics. In this study, several experiments under various conditions were performed to verify the adaptability of Horio's scaling law. The experimental results indicate that the Horio's scaling law can be successfully applied to mixing and segregation behaviors of Geldart B particles in BFB under the conditions studied in this paper if the scaling relations are strictly satisfied, which is expected to be helpful in the design of fluidized bed reactor. (C) 2012 Elsevier B.V. All rights reserved.