Optimal Mobile-Phase Composition for the Resolution of Deoxyribonucleosides in RP-HPLC

Myoung Seup So; Young Kwang Park; Dai Ki Choi; Kyung Ho Row

Journal of Industrial and Engineering Chemistry, Vol.4, No.1, 68-72, March, 1998

Myoung Seup So, Young Kwang Park¹, Dai Ki Choi², and Kyung Ho Row¹

Korea Kumho Petrochemical Co., LTD., Kumho Chemical laboratories, Yuseong, Taejon 305-600, Korea
¹Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Ku, Inchon 402-751, Korea
²Environmental Research Center, Korea Institute of Science and Technology, Cheongryang, Seoul 136-791, Korea

Five deoxyribonucleosides (dCyd, dUrd, dGuo, dThd, and dAdo) were separated by RP-HPLC under isocratic conditions. The volume percent of organic modifier was changed from 3.0 to 10.0 for methanol and acetonitrile, respectively. Potassium phosphate, sodium borate and TADP were used as buffer chemicals. The experimental results showed that for the separation of deoxyribonucleosides methanol was superior to acetonitrile as the organic modifier and the optimum condition of mobile phase was 5.0 vol. % of acetonitrile with 20 mM sodium borate.

[References]

Wu DR, Lohse K, Greenblatt HC, J. Chromatogr., 702, 233 (1995)
McBurney A, Gibson T, Clin. Chim. Acta, 102, 19 (1980)
Carr PW, Li J, Dallas AJ, Eikens DI, Tan LC, J. Chromatogr., 656, 131 (1993)
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Park JH, Tan LC, Li J, Carr PW, Evans JF, J. Chromatogr., 767, 1 (1997)
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Horvath C, Melander W, Molnar I, Anal. Chem., 49, 142 (1977)
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Krstulovic AM, Hartwick RA, Brown PR, J. Chromatogr., 158, 365 (1978)
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Kuo KC, McCune RA, Gehrke CW, Midgett R, Ehrlich M, Nucl. Acids Res., 8, 4763 (1980)
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[Related Articles]

[1] Wu DR, Lohse K, Greenblatt HC, J. Chromatogr., 702, 233 (1995)

[2] McBurney A, Gibson T, Clin. Chim. Acta, 102, 19 (1980)

[3] Carr PW, Li J, Dallas AJ, Eikens DI, Tan LC, J. Chromatogr., 656, 131 (1993)

[4] Carr PW, Tan LC, Park JH, J. Chromatogr., 724, 1 (1996)

[5] Park JH, Tan LC, Li J, Carr PW, Evans JF, J. Chromatogr., 767, 1 (1997)

[6] Molnar I, Horvath C, J. Chromatogr., 145, 371 (1978)

[7] Horvath C, Melander W, Molnar I, Anal. Chem., 49, 142 (1977)

[8] Knox JH, Jurand J, J. Chromatogr., 203, 85 (1981)

[9] Dorsey JG, Khaledi MG, J. Chromatogr., 656, 485 (1993)

[10] Melander WR, 'in High-performance Liquid Chromatography, Advances and Perspectives,' vol. 2, (ed. Cs. Horvath), Academic Press, New York (1989)

[11] Krstulovic AM, Hartwick RA, Brown PR, J. Chromatogr., 158, 365 (1978)

[12] Hartwick RA, Krstulovic AM, Brown PR, J. Chromatogr., 186, 659 (1979)

[13] Assenza SP, Brown PR, J. Chromatogr., 277, 305 (1983)

[14] Kuo KC, McCune RA, Gehrke CW, Midgett R, Ehrlich M, Nucl. Acids Res., 8, 4763 (1980)

[15] Hartwick RA, Assenza SP, Brown PR, J. Chromatogr., 186, 647 (1979)

[16] Agarwal RP, Major PP, Kufe DW, J. Chromatogr., 231, 418 (1982)