Layered lithium titanate whisker, Li_xTi₄O₉ , was prepared by a host-guest reaction [l]. Lithium insertion into the layered lithium titanate whisker was investigated to test the feasibility of its application as an active material for a rechargeable lithium cell. The charge and discharge cycle performances of Li/Li_xTi₄O₉ were measured between 3.0 V and 0.5 V with 0.25 mA/㎠. Using 1 M LiC1O₄ in propylene carbonate(PC) as the electrolyte, the initial discharge capacity of 130 mAh/g was obtained at a plateau region of 1.2V. For the first 20 cycles, the discharge capacity obtained was between 80∼100 mAh/g, then decayed to 60∼80 mAh/g due to a defect in spinel oxides Li₄Ti₅O₁₂.