In this study, methylated soy protein (MeSP) was applied to the solid-liquid separation or flocculation of kaolin and kanto loam (deposits of volcanic ash) suspensions (3.0 g/L). The flocculation performance of MeSP was evaluated in terms of supernatant clarity (jar test method) and floc settling rate (sedimentation balance method), and compared with those of chitosan and polyaluminum chloride (PAC). At pH 7, the optimum dosage of MeSP, chitosan and PAC were almost the same (about 1.5 wt.% of the suspended solids), but there was a remarkable difference in the floc settling rate. At the optimum dosage, the settling rate of the floc formed by MeSP was 6-7 mm/s, while those formed by chitosan and PAC were about 2 mm/s. MeSP showed the highest flocculation performance at pH 3-7 among these flocculants. At pH 8-12, the flocculation performance of MeSP was much higher than that of PAC, but lower than that of chitosan. The flocculation performance of MeSP for kanto loam suspension was strongly affected by the addition of seawater, while the effect of seawater concentration on the flocculation performance of MeSP for kaolin was moderate. (C) 2012 Elsevier B.V. All rights reserved.