The objective of this study was the development and optimization of a method for the liquid-liquid extraction and recovery of Uranium (VI) traces (0.10 mg/L). A comparison was made of three different extracting agents at 0.10 mol/L: tri-n-butyl phosphate, methyltrioctylammonium chloride, and bis(2-ethylhexyl) phosphoric acid. As the optimized chemical conditions of the liquid-liquid systems are very important for the future applications for the extraction, recovery and pre-concentration of Uranium (VI) traces, whether in bulk liquid membranes or in supported liquid membranes systems, a response surface methodology was adopted. A Doehlert design is used to obtain a quadratic function relationship between the percentage of recovery of uranyl ion and the amount of bis(2-ethylhexyl) phosphoric acid in kerosene, as extracting agent (0.001-0.050 mol/L) and that of phosphoric (0.192-0.718 mol/L) and citric (0.206-0.698 mol/L) acids, mixed as recovery agents. From the results, the most important effects on the recovery are the concentration of bis(2-ethylhexyl) phosphoric acid in the organic phase and the concentration of phosphoric acid in the stripping solution, while the concentration of citric acid only favors the extraction of Uranium (VI) when the other compounds are at opposite levels of concentration. From the mathematical model, optimal conditions for the back-extraction of Uranium (VI) were found with predicted recoveries of 99%, which were later corroborated experimentally. (C) 2013 Elsevier B.V. All rights reserved.