Two extracellular peroxidases from Phanerochaete chtysosporium, named lignin peroxidase (UP) and manganese peroxidase (MnP), were extracted simultaneously by biosurfactant-based reversed micellar extraction method. Rhamnolipid (RL) was applied during the experiment. N-hexanol was chosen for the extraction from five different co-solvents including n-butanol, n-pentanol, n-hexanol, n-heptanol, and n-octanol. Parameters (RL concentration, salt concentration, pH, temperature, extraction time, and ethanol dosage) in forward and backward extraction process were investigated. Under the optimum conditions, the highest activity recoveries of LiP and MnP were up to 93.56% and 88.79%, respectively. This observation may be helpful in developing a more effective method to monitor the process of synchronous extraction of different enzymes by reversed micellar extraction. (C) 2013 Elsevier B.V. All rights reserved.