The removal of Pb(II) ion from our environment especially waste water is now shifting from the use of conventional adsorbents to the use of biosorbents. The presence of heavy metals in the environment is of major concern because of their toxicity and threat to human life and the environment. A new type of Pb(II) ion imprinted alginic acid interpenetrating polymer networks (IPN) were prepared for Pb(II) ion removal by ion imprinting strategy. The Pb(II) ion imprinted alginate and N,N'-methylene-bis-acrylamide(NNMBA)-crosslinked polyacrylamide based IPN were synthesized with acrylamide in the presence of alginic acid. The influence of matrix on the sorption efficiency for Pb(II) ion and selectivity against metal ions such as Zn(II), Cu(II), and Cd(II) ions on IPN were described. The structure and morphology of Pb(II) ion imprinted IPN were followed by various analytical methods. The separation characteristics of the IPN for Pb(II) ion were investigated by batch and column procedures and analyzed by AAS. Based on the packed columns with Pb(II) ion imprinted IPN, a highly selective sorbent for Pb(II) ion from aqueous solution was developed by the imprinting approach and the developed polymer could be used for the selective sorption of trace amounts of Pb(II) ions from coexisting metal ions and from waste water.