Some strains of Rhodosporidium toruloides can produce high concentrations of single cell oil. However, this oleaginous yeast does not produce amylases. Cells of the amylase-producing yeast Saccharomycopsis fibuligera All-c were immobilized using polyvinyl alcohol. The immobilized yeast could produce 325 U/ml of amylase activity within 72 h of incubation. These amylases hydrolyzed cassava starch and the resulting product was converted into single cell oil by R. toruloides 21167. In a 2-1 co-culture bioreactor, a single cell oil yield (64.9% w/w) from a cell mass of R. toruloides 21167 (20.1 g/1) were produced from cassava starch (6.0% w/v). Over 96% of the fatty acids produced were C-16:0, C-18:0, C-58:1 and C-18:2, useful for conversion into biodiesel. (C) 2013 Elsevier Ltd. All rights reserved.