Solubility of mitomycin C drug in ethanol-water solutions was measured at different temperatures (0-50 degrees C) using high performance liquid chromatography. It was observed that the solubility of mitomycin C increases with temperature up to certain concentration of ethanol-water, after that it reduces due to several solute-solvent actions. The process is quite useful for developing nano-crystals of mitomycin C by changing the concentration of co-solvent mixture. From the observations of the present study, it may be suggested that for cardiovascular, ophthamalic and cancer applications of mitomycin C one can use ethanol-water co-solvent mixture for deriving nano-crystals. A modified Apelblat equation was used to correlate the experimental data. (C) 2012 Elsevier B.V. All rights reserved.