In the present article, we have synthesized CoFe/Au/CoFe multisegment nanowires by electrochemical deposition technique and selectively functionalized the surface of Au segment with cysteamine for biological and chemical applications. The morphological structure was examined by scanning electron microscope and found that the nanowires are around 4 mu m in length. The magnetic property of the nanowires was measured by vibrating sample magnetometer. We have compared the magnetic properties of CoFe/Au/CoFe multisegment nanowires with CoFe nanowires and found that there is a decrease in magnetic moment for multisegment nanowires. The Au segment of the fabricated multisegment nanowires is selectively functionalized with cysteamine. The average composition and the surface functionalization of nanowires were confirmed through electron dispersive spectroscopy and X-ray photoelectron spectroscopy. (C) 2013 Elsevier B.V. All rights reserved.